2025
Differential Analysis of Gene Expression in Severely Sick COVID-19 Patients
Rodríguez-Carrascal, D., Hernández, F., & Ramírez, C. D.
International Journal of Advanced Research, 710–714
DOI: 10.21474/IJAR01/20950Daniela
Rodríguez Carrascal
Biology graduate and PhD candidate in Pharmacy & Biochemistry, with hands-on experience in research and development, protocol standardization, and technical report generation. I have worked in biotech/pharma environments in development and scale-up activities, characterization, analytical techniques, quality control (HPLC/FPLC, stability-associated routines, and procedure-based documentation). I focus on traceability, reproducibility, data quality, and clear documentation, transforming complex experimental work into structured, industry-applicable results.
Experience / Expertise
Biology graduate and PhD candidate in Pharmacy & Biochemistry, with hands-on experience in research and development, protocol standardization, and technical report generation. I have worked in biotech/pharma environments in development and scale-up activities, characterization, analytical techniques, quality control (HPLC/FPLC, stability-associated routines, and procedure-based documentation). I focus on traceability, reproducibility, data quality, and clear documentation, transforming complex experimental work into structured, industry-applicable results.
8+
Publications
5+
Institutions
8+
Years Research
Areas of Expertise
R&D and translational development (cell therapy · nanotechnology · neuroregeneration)Cell culture and bioassays (stem cells · Schwann cells · cell lines)Analytics and characterization (HPLC/FPLC · ELISA · Western blot · protein quantification)Quality control and stability (raw materials · intermediates · final product · stability support)Documentation and compliance (GLP/GMP · protocols · technical reports · regulatory documentation)
Work Experience
2023 – Present
Doctoral Researcher
Multipotent Cells in Regeneration Laboratory
Institute of Biological Chemistry and Physicochemistry (IQUIFIB). Faculty of Pharmacy and Biochemistry, University of Buenos Aires (UBA) / CONICET · Argentina
- • Doctoral research focused on peripheral nerve regeneration through cell therapy and nanotechnology.
- • Culture of stem cells, Schwann cells, and neuroblastoma cell lines.
- • Animal model management and experimental surgeries.
- • Cell transplantation and administration of nanoformulated drugs.
- • Applied techniques: histology, microscopy, PCR, Western blot, and protein quantification.
- • Work according to ethical regulations, Good Laboratory Practices (GLP), and scientific documentation standards.
2022 – 2023
R&D Analyst
Biotecfar S.A.
Venezuela
- • Development and scale-up of immunobiologicals from pilot stage to larger-scale production.
- • Protein and antibody purification via HPLC, FPLC, electrophoresis, filtration, ultrafiltration, and centrifugation.
- • Quantification and characterization by ELISA, Western blot, and associated analytical techniques.
- • Quality control of raw materials, intermediate, and final products.
- • Analytical validation, stability studies, and regulatory compliance.
- • Application of GLP and GMP in development, production, and technical documentation.
- • In vitro and in vivo assays for efficacy and biological safety evaluation.
- • Writing of technical reports, protocols, and regulatory documentation.
- • Mentoring undergraduate students in laboratory techniques and research projects.
2017 – 2022
Research Assistant
Genetic and Forensic Studies Unit
Venezuelan Institute for Scientific Research (IVIC), Venezuela
- • Biological sample processing for genetic and forensic analyses (paternity and identification).
- • Application of molecular biology techniques: DNA/RNA purification, PCR/qPCR, and sequencing support (Sanger / Illumina MiSeq).
- • Sequence and genetic variant analysis using bioinformatics tools (in R).
- • RNA-seq data analysis, differential gene expression, and gene ontology (GO) enrichment.
- • Standardization of Sanger sequencing with SNaPshot kit for KRAS/NRAS mutation detection (colorectal cancer).
- • Statistical correlation between genetic mutations and histopathological parameters.
2017
Research Assistant
Molecular Biology Laboratory
Institute of Tropical Medicine, Central University of Venezuela (UCV), Venezuela
- • Standardization of an isoenzymatic method for Trypanosoma cruzi typing.
- • Extraction, electrophoresis, and protein analysis (laboratory work and result recording).
- • Parasite culture and result validation through positive and negative controls.
2017
Intern
Molecular Diagnostics Unit
Jacinto Convit Foundation, Venezuela
- • Molecular diagnosis of infectious diseases and neoplasms.
- • Application of conventional PCR, real-time PCR (qPCR), and pyrosequencing.
- • Clinical sample processing and diagnostic result validation.
- • Work under biosafety standards and quality control procedures.
Available for new projects
Services
Specialized scientific support for academic groups, early-stage biotechs, CROs, and pharma R&D teams. Reproducible deliverables, ready for publication or regulatory audit — in English or Spanish, regardless of geography.
Bioinformatics & Omics Data Analysis
Bulk RNA-seq · single-cell · reproducible workflows
End-to-end omics data analysis: bulk and single-cell RNA-seq, differential expression, functional enrichment (GSEA, KEGG, Reactome), and publication-ready visualizations (UMAP, PCA, volcano plots, heatmaps). Reproducible workflows in Nextflow/nf-core for theses, papers, posters, and technical reports.
RNA-seqscRNA-seqnf-coreAnalytical Method Development & Validation
HPLC · FPLC · ELISA · Western blot
Fit-for-purpose analytical validation packages aligned with ICH Q2(R2)/Q14: protocol, execution, report, and data integrity to support IND/IMPD submissions or product release.
ICH Q2(R2)ICH Q14HPLCFPLCELISAWestern blotLOD/LOQSpecificityCMC & Quality Documentation
SOPs · CAPA · stability · tech transfer
Audit-ready CMC and quality documentation for virtual biotechs and scaling labs: SOPs, batch records, deviations/CAPA, stability studies, and technical dossiers.
GLPICH Q1AALCOA+Data integrityStabilityScientific & Regulatory Writing
Manuscripts · IND/IMPD · response to reviewers
Specialized writing and editing in biomedicine and biotechnology: papers, IND/IMPD sections, investigator's brochures, response letters, and editor correspondence in English and Spanish.
IND/IMPDPeer reviewES↔ENBiomedicineExperimental Design & Assay Strategy
DoE · QbD · power analysis · fit-for-purpose
Experimental strategy advisory for R&D teams and PIs: study design, sample-size calculation, batch-effect mitigation, and assay selection aligned with program phase.
DoEQbDPower analysisFit-for-purposeRisk-basedSample sizeGrant & Funding Proposals
NIH · ERC · Horizon Europe · MSCA · Wellcome
Writing and review of international and regional funding proposals. Aims, approach, impact, work plan, and budget justification crafted for peer-review scoring.
Lab Notebook & ELN Audit
Pre-defense · pre-audit · handoff
Audit and reorganization of lab notebooks (paper or electronic) ahead of defenses, regulatory inspections, or project handover. ALCOA+ traceability and GLP rigor.
ALCOA+ELNGLPPre-auditHandoffReproducibility
How I work
- Mode: 100% remote · timezone UTC−3 (Buenos Aires) · async-friendly
- Delivery languages: English and Spanish · bilingual documents at no extra cost
- Format: fixed-scope packages, per project/milestone, hourly, or monthly retainer
- Free initial scoping call to align scope, deliverables, and deadline
- Honest scope: I don't ghostwrite theses and won't co-author papers I didn't take part in
Skills
Education
PhD Candidate
Faculty of Pharmacy and Biochemistry, University of Buenos Aires (UBA-CONICET)
Nov 2023 – Present
Bachelor's in Biology (Cell and Molecular Biology Specialization)
Central University of Venezuela (UCV)
Completed 2022
Research and Development
- Experimental design and execution, protocol standardization, reproducibility and traceability
- Analysis and interpretation of results; technical/scientific reports
Cell culture, models and experimental techniques
- Stem cell and cell line culture; biosafety and good practices
- Work with experimental models (in vivo/in vitro per protocol)
Nanotechnology and extracellular vesicles
- Applied nanotechnology approaches and extracellular vesicle workflows
- Optimization/standardization of experimental processes
Analytics, proteins and QC support
- HPLC / FPLC, downstream workflows and associated documentation
- ELISA · Western blot · SDS-PAGE, protein quantification
- Stability support and QC routines (raw materials/intermediates/final)
Molecular biology and bioinformatics (prior experience)
- DNA/RNA, PCR/qPCR, Sanger / Illumina MiSeq
- R, RNA-seq, differential expression and GO enrichment
Clinical and data (remote)
- Clinical data entry and record verification · HIPAA compliance
Tools
- R, GraphPad Prism, ImageJ, Excel/Google Sheets, MS Office/Google Workspace
Soft Skills
- Technical communication, teamwork, organization, attention to detail, problem solving
Institutions
Affiliations
- • International Society for Neurochemistry (ISN) — Member
Languages
Spanish (native)English (advanced)Portuguese (intermediate)
Complementary Training (relevant selection)
- HPLC Update Program — College of Pharmacists and Biochemists (2025)
- Single Cell – Latin America and the Caribbean — University of Costa Rica · Wellcome Connecting Science (Jul 2025)
- Pharmaceutical Technology I — Faculty of Pharmacy and Biochemistry, UBA (Mar–Jul 2025)
- Advanced Course in Extracellular Vesicles — Universidad Francisco de Vitoria, Madrid (Jan–Apr 2025)
- Clinical Study Monitoring — "Luis M. Zieher" Foundation (Jan 2025)
- Update in Analytical Methods — UBA (Apr–Jul 2024)
- Fluorescence Microscopy — UBA (Jun 2024)
- Mutagenesis and Functional Characterization of Proteins in Eukaryotic Cells — UBA (Dec 2023)
Projects
Research on new therapeutic approaches for peripheral nervous system injuries
PhD, Institute of Biological Chemistry and Physicochemistry (IQUIFIB)
Cell-based, nanotechnology, and pharmaceutical reformulation approaches. R&D project oriented to regenerative strategies focused on experimental standardization, reproducibility, and traceability, translating complex work into structured results.
R&DTranslationalDocumentationStandardization
Extracellular vesicles
PhD, Institute of Biological Chemistry and Physicochemistry (IQUIFIB)
Research workflows with sample processing and protein-related analyses, with protocol optimization and consistent reporting.
EVsProteinsReportingReproducibility
Development and scale-up of immunobiologicals and quality control analysis
Biotecfar
Development/scale-up, purification and characterization, with analytical support (HPLC/FPLC), stability, and technical documentation.
DevelopmentDownstreamHPLCFPLCStabilityQC
Molecular diagnostics and bioinformatics
Venezuelan Institute for Scientific Research
PCR/qPCR workflows, sequencing, RNA-seq, and R analysis with structured reporting.
Molecular DiagnosticsBioinformaticsRNA-seqR
Peer-reviewed Publications
2025
RAS Oncogenes in Venezuelan Colorectal Cancer: Detection Methods and Mutational Profile Review
Ramírez, C. D., et al.
Academia Oncology
DOI: 10.20935/AcadOnco77912025
CRISPR-Cas Systems: Origins and their Impact on Functional Genomic
Rodríguez-Carrascal, D.
Premier Journal of Science, 8:100069
DOI: 10.70389/PJS.1000692025
Stem Cells in Oncology: Potential and Challenges in Cancer Therapies
Rodríguez-Carrascal, D.
Premier Journal of Science, 6:100053
DOI: 10.70389/PJS.1000532025
Biomarkers: Key to Precision Therapy for Cancer
Rodríguez-Carrascal, D.
Premier Journal of Science, 7:100057
DOI: 10.70389/PJS.1000572024
Evaluation of genetic methods for molecular diagnosis in colorectal cancer, with applications in personalized medicine
Rodríguez-Carrascal, D., et al.
ecancer
2022
Highlights of the 1st Ecuadorian-Venezuelan Symposium of Young Researchers in Bioinformatics (1SEVJIB)
Ayala-Ruano, S., Hernández, F., Ortega, A., Infante, D., Carrascal, D., Sánchez-Luquez, K., & Puche-Quiñonez, R.
F1000Research, 11:1086
DOI: 10.12688/f1000research.125381.12020
Evaluación molecular de los genes KRAS y NRAS en biopsias de pacientes con cáncer colorrectal
Ramírez, C., & Rodríguez, D.
Rev. Fac. Med. (UCV), 43(Supl. 1)