Differential Analysis of Gene Expression in Severely Sick COVID-19 Patients

Q: What is Daniela Rodríguez Carrascal's research focus?

Daniela specializes in cell biology and translational research, with expertise in cell culture, nanotechnology, extracellular vesicles, and peripheral nerve regeneration.

Q: What laboratory techniques does Daniela have experience with?

Daniela has extensive experience with HPLC, FPLC, ELISA, Western blot, PCR, cell culture, flow cytometry, immunofluorescence, and molecular biology techniques.

Q: Where is Daniela pursuing her PhD?

Daniela is a PhD candidate at Universidad de Buenos Aires, Faculty of Pharmacy & Biochemistry, conducting research at the Instituto de Química y Fisicoquímica Biológicas (IQUIFIB, UBA-CONICET).

Rodríguez Carrascal, D., Hernández, F., & Ramírez, C. D.; Ramírez, C. D., et al.; Rodríguez Carrascal, D.; Rodríguez Carrascal, D.; Rodríguez Carrascal, D.; Rodríguez Carrascal, D., et al.; Ayala-Ruano, S., Hernández, F., Ortega, A., Infante, D., Carrascal, D., Sánchez-Luquez, K., & Puche-Quiñonez, R.; Ramírez, C., & Rodríguez, D.

doi:10.21474/IJAR01/20950

Daniela
Rodríguez Carrascal

Biology graduate and PhD candidate in Pharmacy & Biochemistry, with hands-on experience in research and development, protocol standardization, and technical report generation. I have worked in biotech/pharma environments in development and scale-up activities, characterization, analytical techniques, quality control (HPLC/FPLC, stability-associated routines, and procedure-based documentation). I focus on traceability, reproducibility, data quality, and clear documentation, transforming complex experimental work into structured, industry-applicable results.

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Experience / Expertise

8+

Publications

5+

Institutions

8+

Years Research

Areas of Expertise

R&D and translational development (cell therapy · nanotechnology · neuroregeneration)Cell culture and bioassays (stem cells · Schwann cells · cell lines)Analytics and characterization (HPLC/FPLC · ELISA · Western blot · protein quantification)Quality control and stability (raw materials · intermediates · final product · stability support)Documentation and compliance (GLP/GMP · protocols · technical reports · regulatory documentation)

Work Experience

2023 – Present

Doctoral Researcher

Multipotent Cells in Regeneration Laboratory

Institute of Biological Chemistry and Physicochemistry (IQUIFIB). Faculty of Pharmacy and Biochemistry, University of Buenos Aires (UBA) / CONICET · Argentina

• Doctoral research focused on peripheral nerve regeneration through cell therapy and nanotechnology.
• Culture of stem cells, Schwann cells, and neuroblastoma cell lines.
• Animal model management and experimental surgeries.
• Cell transplantation and administration of nanoformulated drugs.
• Applied techniques: histology, microscopy, PCR, Western blot, and protein quantification.
• Work according to ethical regulations, Good Laboratory Practices (GLP), and scientific documentation standards.

2022 – 2023

R&D Analyst

Biotecfar S.A.

Venezuela

• Development and scale-up of immunobiologicals from pilot stage to larger-scale production.
• Protein and antibody purification via HPLC, FPLC, electrophoresis, filtration, ultrafiltration, and centrifugation.
• Quantification and characterization by ELISA, Western blot, and associated analytical techniques.
• Quality control of raw materials, intermediate, and final products.
• Analytical validation, stability studies, and regulatory compliance.
• Application of GLP and GMP in development, production, and technical documentation.
• In vitro and in vivo assays for efficacy and biological safety evaluation.
• Writing of technical reports, protocols, and regulatory documentation.
• Mentoring undergraduate students in laboratory techniques and research projects.

2017 – 2022

Research Assistant

Genetic and Forensic Studies Unit

Venezuelan Institute for Scientific Research (IVIC), Venezuela

• Biological sample processing for genetic and forensic analyses (paternity and identification).
• Application of molecular biology techniques: DNA/RNA purification, PCR/qPCR, and sequencing support (Sanger / Illumina MiSeq).
• Sequence and genetic variant analysis using bioinformatics tools (in R).
• RNA-seq data analysis, differential gene expression, and gene ontology (GO) enrichment.
• Standardization of Sanger sequencing with SNaPshot kit for KRAS/NRAS mutation detection (colorectal cancer).
• Statistical correlation between genetic mutations and histopathological parameters.

2017

Research Assistant

Molecular Biology Laboratory

Institute of Tropical Medicine, Central University of Venezuela (UCV), Venezuela

• Standardization of an isoenzymatic method for Trypanosoma cruzi typing.
• Extraction, electrophoresis, and protein analysis (laboratory work and result recording).
• Parasite culture and result validation through positive and negative controls.

2017

Intern

Molecular Diagnostics Unit

Jacinto Convit Foundation, Venezuela

• Molecular diagnosis of infectious diseases and neoplasms.
• Application of conventional PCR, real-time PCR (qPCR), and pyrosequencing.
• Clinical sample processing and diagnostic result validation.
• Work under biosafety standards and quality control procedures.

Skills

Education

PhD Candidate

Faculty of Pharmacy and Biochemistry, University of Buenos Aires (UBA-CONICET)

Nov 2023 – Present

Bachelor's in Biology (Cell and Molecular Biology Specialization)

Central University of Venezuela (UCV)

Completed 2022

Research and Development

Experimental design and execution, protocol standardization, reproducibility and traceability
Analysis and interpretation of results; technical/scientific reports

Cell culture, models and experimental techniques

Stem cell and cell line culture; biosafety and good practices
Work with experimental models (in vivo/in vitro per protocol)

Nanotechnology and extracellular vesicles

Applied nanotechnology approaches and extracellular vesicle workflows
Optimization/standardization of experimental processes

Analytics, proteins and QC support

HPLC / FPLC, downstream workflows and associated documentation
ELISA · Western blot · SDS-PAGE, protein quantification
Stability support and QC routines (raw materials/intermediates/final)

Molecular biology and bioinformatics (prior experience)

DNA/RNA, PCR/qPCR, Sanger / Illumina MiSeq
R, RNA-seq, differential expression and GO enrichment

Clinical and data (remote)

Clinical data entry and record verification · HIPAA compliance

Tools

R, GraphPad Prism, ImageJ, Excel/Google Sheets, MS Office/Google Workspace

Soft Skills

Technical communication, teamwork, organization, attention to detail, problem solving

Institutions

Affiliations

• International Society for Neurochemistry (ISN) — Member

Languages

Spanish (native)English (advanced)Portuguese (intermediate)

Complementary Training (relevant selection)

HPLC Update Program — College of Pharmacists and Biochemists (2025)
Single Cell – Latin America and the Caribbean — University of Costa Rica · Wellcome Connecting Science (Jul 2025)
Pharmaceutical Technology I — Faculty of Pharmacy and Biochemistry, UBA (Mar–Jul 2025)
Advanced Course in Extracellular Vesicles — Universidad Francisco de Vitoria, Madrid (Jan–Apr 2025)
Clinical Study Monitoring — "Luis M. Zieher" Foundation (Jan 2025)
Update in Analytical Methods — UBA (Apr–Jul 2024)
Fluorescence Microscopy — UBA (Jun 2024)
Mutagenesis and Functional Characterization of Proteins in Eukaryotic Cells — UBA (Dec 2023)

Projects

Research on new therapeutic approaches for peripheral nervous system injuries

PhD, Institute of Biological Chemistry and Physicochemistry (IQUIFIB)

Cell-based, nanotechnology, and pharmaceutical reformulation approaches. R&D project oriented to regenerative strategies focused on experimental standardization, reproducibility, and traceability, translating complex work into structured results.

R&DTranslationalDocumentationStandardization

Extracellular vesicles

PhD, Institute of Biological Chemistry and Physicochemistry (IQUIFIB)

Research workflows with sample processing and protein-related analyses, with protocol optimization and consistent reporting.

EVsProteinsReportingReproducibility

Development and scale-up of immunobiologicals and quality control analysis

Biotecfar

Development/scale-up, purification and characterization, with analytical support (HPLC/FPLC), stability, and technical documentation.

DevelopmentDownstreamHPLCFPLCStabilityQC

Molecular diagnostics and bioinformatics

Venezuelan Institute for Scientific Research

PCR/qPCR workflows, sequencing, RNA-seq, and R analysis with structured reporting.

Molecular DiagnosticsBioinformaticsRNA-seqR

Peer-reviewed Publications

2025